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Re: [ccp4bb] Off topic: where to order just miniprep columns?

 

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CCP4bb <-- 2007 <-- July 2007 <-- 05 July 2007
Previous message:
Subject: How to remove nucleic acid contamination for crystallizing zinc finger protein
From: tony_htc {- at -} 126 {- dot -} COM tony_htc {- at -} 126 {- dot -} COM
Date: 2007-07-05
Next message:
Subject: Re: How to remove nucleic acid contamination for crystallizing zinc finger protein
From: Ana Silva silva {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-07-05


Subject: Re: Off topic: where to order just miniprep columns?
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2007-07-05

There are several sources.

You can often get them from the manufacturer of your miniprep kit
(the catalog # is written on the side of the bag they come in within
the kit). Many times, they don't list in their catalog that they
will sell it separately, but you can call and get them to give you a
price and such. This also goes for the individual bottles of reagent
that come in the kit (useful when somebody knocks a bottle over). I
have found that the bulk of the price of the miniprep kit is in the
spin columns, so you won't save much money getting spin columns from,
say, Qiagen.

For third party manufacturers we have used the tubes from UPrep
(http://www.uprepusers.com/) and they have worked fine.

I've also seen the tubes (from a different third party manufacturer)
advertised on eBay, but can't speak to their quality.

Best,

Cynthia


On Jul 4, 2007, at 1:33 PM, D. Eric Dollins wrote:

> Hello,
> Sorry for the off-topic question.....Does anyone know a way to just
> buy the spin columns from the Qiagen (or similar) PCR clean up / Gel
> extraction / miniprep kits, without buying the entire kit? Our lab is
> deluged with buffer components but seem to be perpetually running out
> of the columns.
> Thanks
> Eric
>
>
>
> --
> D. Eric Dollins, Ph.D.
> C266 LSRC, Research Dr.
> Duke University Medical Center
> Durham, NC 27710
> (919) 681-1668, d.e.dollins@gmail.com

____________________
Cynthia Kinsland, Ph.D.
Cornell University
Protein Facility Director
607-255-8844




CCP4bb navigation

CCP4bb <-- 2007 <-- July 2007 <-- 05 July 2007
Previous message:
Subject: How to remove nucleic acid contamination for crystallizing zinc finger protein
From: tony_htc {- at -} 126 {- dot -} COM tony_htc {- at -} 126 {- dot -} COM
Date: 2007-07-05
Next message:
Subject: Re: How to remove nucleic acid contamination for crystallizing zinc finger protein
From: Ana Silva silva {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-07-05



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