[ccp4bb] Solubility of ligands

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CCP4bb <-- 2007 <-- February 2007 <-- 28 February 2007

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From: vonrhein {- at -} GLOBALPHASING {- dot -} COM
Date: 2007-02-28

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Subject: Re: Solubility of ligands
From: knettles {- at -} SCRIPPS {- dot -} EDU
Date: 2007-02-28

Subject: Solubility of ligands
From: I {- dot -} moustafa {- at -} PSU {- dot -} EDU
Date: 2007-02-28

Dear all,

I have a small library of In-silico screened compounds to test for
activity and for crystallization trials with our protein of interest.

We only have about 10 mg/ml of each compound. As there is no
available experimental information about solubility of these
compounds, I have no choice but to try different solvents.

The first solvent to try will be DMSO (100%) to make the highest
stock concentration of each compound. My question to those who passed
through similar experience is:

Assuming some of the compounds turned to be insoluble in DMSO,
which is possible, how to completely recover the compounds from DMSO
before trying another solvent.

Will spinning and leaving the tube open over the bench be enough
to get rid of the solvent or what people usually do in that case?
What is the recommended solvent to try next?

Is there a standard protocol to follow for the case we have??

thanks in advance for those who are willing to share their experience.

regards,
Ibrahim

Ibrahim M.Moustafa, Ph.D.
Pennsylvania State University
Biochemistry & Molecular Biology Dept.
201 Althouse Lab.
University Park, PA16802

Tel (814) 863 8703
Fax (814) 865 7927

CCP4bb navigation

CCP4bb <-- 2007 <-- February 2007 <-- 28 February 2007

Previous message:
Subject: Re: process SeMet labelled data
From: vonrhein {- at -} GLOBALPHASING {- dot -} COM
Date: 2007-02-28

Next message:
Subject: Re: Solubility of ligands
From: knettles {- at -} SCRIPPS {- dot -} EDU
Date: 2007-02-28