[ccp4bb] needles vs crystals

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2007 <-- August 2007 <-- 07 August 2007

Previous message:
Subject: Re: Surface complementarity
From: "Rizkallah, PJ (Pierre)" p {- dot -} j {- dot -} rizkallah {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-08-07

Next message:
Subject: Re: needles vs crystals
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2007-08-07

Subject: needles vs crystals
From: shivesh kumar 2shivesh {- at -} GMAIL {- dot -} COM
Date: 2007-08-07

Dear all,
I am trying to crystallize a 7kDa protein with MPD as a precipitant.I got
the needles with precipitation in two days at 30% and in 4-5 days,needles
appear in all the drops(pH 3.8-4.8).The concentration of protein is around
20mg/ml and the volume of mother liquor in 500microlt.What should I do to
get good crystals,the temp. I am trying is 16C.Thanx in advance for the
suggestions.
Shivesh Kumar

CCP4bb navigation

CCP4bb <-- 2007 <-- August 2007 <-- 07 August 2007

Previous message:
Subject: Re: Surface complementarity
From: "Rizkallah, PJ (Pierre)" p {- dot -} j {- dot -} rizkallah {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-08-07

Next message:
Subject: Re: needles vs crystals
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2007-08-07