[ccp4bb] Data collection of SAD (MAD) data of Copper-containing protein

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CCP4bb <-- 2007 <-- March 2007 <-- 15 March 2007

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Subject: Commensurate superlattices in protein crystallography?
From: John R Helliwell jrhelliwell {- at -} GMAIL {- dot -} COM
Date: 2007-03-15

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Subject: Re: Data collection of SAD (MAD) data of Copper-containing protein
From: ES manian1 {- at -} VSNL {- dot -} COM
Date: 2007-03-15

Subject: Data collection of SAD (MAD) data of Copper-containing protein
From: Yi Xue yi-xue {- at -} NORTHWESTERN {- dot -} EDU
Date: 2007-03-15

Dear all:
We already got nice crystals of a drug-protein complex, however, MR
failed due to the huge copies (>12) of protein molecules per asu. Protein
itself is a small one, only ~70 aa.
Later on, we collected MAD data of copper (copper : protein ~ 1: 1),
Rsym of the data was around ~9%, the anomalous signals were weak, and only
good to ~6A, the data also failed to solve the phases.
Thus, basically, the Cu anomallous signal is very weak, and the
crystals are kind of sensitive to radiation, it is dying but not that fast.
I am wondering, Do we stand any chances to solve the phases by Cu MAD
or SAD?

Any suggestions or comments are highly appreciated?
Yi

CCP4bb navigation

CCP4bb <-- 2007 <-- March 2007 <-- 15 March 2007

Previous message:
Subject: Commensurate superlattices in protein crystallography?
From: John R Helliwell jrhelliwell {- at -} GMAIL {- dot -} COM
Date: 2007-03-15

Next message:
Subject: Re: Data collection of SAD (MAD) data of Copper-containing protein
From: ES manian1 {- at -} VSNL {- dot -} COM
Date: 2007-03-15