[ccp4bb] Question regards to binding affinity of the protein complex?

Protein crystallography Programs for crystallography X-ray detectors
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Protein Peptide

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CCP4bb <-- 2007 <-- September 2007 <-- 19 September 2007

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Subject: Question regards to binding affinity of the protein complex?
From: Ngo Duc Tri ngoductri {- at -} GMAIL {- dot -} COM
Date: 2007-09-19

Dear CCP4 Users,
I'd like to solve the structure of the protein-protein complex. I
intend to purify and incubate the complex then run gel-filtration
before setting crystal.
I'd like to know your experience about the Kd value of the interaction
in order to get the complex after running the gel-filtration. In other
words, what is the lowest Kd that we can purify the protein complex
using gel-filtration?
And if the binding affinity is too low to purify the complex, is it
possible to get the crystal of the complex if I just incubate and set
crystal of this mixture?

Thank you very much for all of your advice!
My best regards,
TriNgo
Sungkyunkwan University

CCP4bb navigation

CCP4bb <-- 2007 <-- September 2007 <-- 19 September 2007

Previous message:
Subject: Summary: pymol movies scripts
From: Sebastiano Pasqualato sebastiano {- dot -} pasqualato {- at -} IFOM-IEO-CAMPUS {- dot -} IT
Date: 2007-09-19

Next message:
Subject: Archiving onto DVD with RH linux
From: "david lawson (JIC)" david {- dot -} lawson {- at -} BBSRC {- dot -} AC {- dot -} UK
Date: 2007-09-19