[ccp4bb] How to reduce no. of overlaps

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Zero occupancy and bad geometry
From: Eleanor Dodson eleanor {- dot -} dodson {- at -} YORK {- dot -} AC {- dot -} UK
Date: 2012-03-07

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Subject: Re: How to reduce no. of overlaps
From: Frank von Delft frank {- dot -} vondelft {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2012-03-07

Subject: How to reduce no. of overlaps
From: Dipankar Manna dipankar_m {- at -} AURIGENE {- dot -} COM
Date: 2012-03-07

Dear Crystallographers,

I am working on a protein having SG P6, the cell parameters are a= 79, b= 79, c= 325. The crystals are forming in big size and with very good shape. It also diffracting very well in Home source facility both in terms of resolution and intensity. But the only problem is the number of overlaps. Its showing much more than the good spots. As a result the completeness is showing maximum up to 65% even after collecting 180 degrees. I am unable to get a complete data. I tried with reducing the oscillation angel to 0.3 degree/0.5 degree but it did not improve that much. Please give me some suggestions.

Regards,

Dipankar

Dipankar Manna
Aurigene Discovery Technologies Limited,
#39-40 KIADB Industrial Area, Electronic City, Phase II,
Hosur Road, Bangalore- 560 100, India
Cell: +91-9538631469
Office Ph : +91 80-66204422 (Extn: 398)
Email ID: dipankar_m@aurigene.com

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Zero occupancy and bad geometry
From: Eleanor Dodson eleanor {- dot -} dodson {- at -} YORK {- dot -} AC {- dot -} UK
Date: 2012-03-07

Next message:
Subject: Re: How to reduce no. of overlaps
From: Frank von Delft frank {- dot -} vondelft {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2012-03-07