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Re: [ccp4bb] How to reduce no. of overlaps

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: setting occupancy for disordered water
From: Herman {- dot -} Schreuder {- at -} SANOFI {- dot -} COM Herman {- dot -} Schreuder {- at -} SANOFI {- dot -} COM
Date: 2012-03-07
Next message:
Subject: FW: endotoxin removal
From: Jerry McCully for-crystallizaiton {- at -} HOTMAIL {- dot -} COM
Date: 2012-03-07


Subject: Re: How to reduce no. of overlaps
From: "Bosch, Juergen" jubosch {- at -} JHSPH {- dot -} EDU
Date: 2012-03-07

In addition to all the excellent suggestions if you can you can also move your detector away from the center of the beam aka increase the detector size in one dimension. Not sure if you can do that at your home source though. By moving the center of the beam say to the lower 9/10 of the detector you will still have the beam position on the detector but you will also have increased the total area. Needless to say you will need to collect over a larger wedge to get a complete dataset.

Regarding your overlap issues, it does help to spend some time thinking about the experiment before collecting the data, I would like to point toward the direction of Mosflm & strategy in conjunction with the Testgen option.

Depending how badly your overlapped data is, you might want to run it through XDS and see how much it can rescue, assuming you used Mosflm or HKL2000. You can also give the Separation Close command a try in Mosflm with Postref Width and Profile Optimize (RTFM for more details).

Jürgen
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-2926
http://web.mac.com/bosch_lab/





CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: setting occupancy for disordered water
From: Herman {- dot -} Schreuder {- at -} SANOFI {- dot -} COM Herman {- dot -} Schreuder {- at -} SANOFI {- dot -} COM
Date: 2012-03-07
Next message:
Subject: FW: endotoxin removal
From: Jerry McCully for-crystallizaiton {- at -} HOTMAIL {- dot -} COM
Date: 2012-03-07



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