[ccp4bb] FW: endotoxin removal

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: How to reduce no. of overlaps
From: "Bosch, Juergen" jubosch {- at -} JHSPH {- dot -} EDU
Date: 2012-03-07

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Subject: Re: How to reduce no. of overlaps
From: "Edward A {- dot -} Berry" BerryE {- at -} UPSTATE {- dot -} EDU
Date: 2012-03-07

Subject: FW: endotoxin removal
From: Jerry McCully for-crystallizaiton {- at -} HOTMAIL {- dot -} COM
Date: 2012-03-07

Dear All;

We purified a His tag protein by Ni-NTA and gel-filtration from E.coli.

We tried two endotoxin removal resins from Pierce. However, it is hard to remove the endotoxins in the purified protein because the protein bound to the resin as well.

This protein contains quite a few aromatic residues and has a pI around 6.

Any ideas to remove the endotoxins will be highly appreciated.

best regards,

Jerry McCully

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: How to reduce no. of overlaps
From: "Bosch, Juergen" jubosch {- at -} JHSPH {- dot -} EDU
Date: 2012-03-07

Next message:
Subject: Re: How to reduce no. of overlaps
From: "Edward A {- dot -} Berry" BerryE {- at -} UPSTATE {- dot -} EDU
Date: 2012-03-07