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Re: [ccp4bb] FW: endotoxin removal

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
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From: Roger Rowlett rrowlett {- at -} COLGATE {- dot -} EDU
Date: 2012-03-07
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From: jens Preben Morth j {- dot -} p {- dot -} morth {- at -} NCMM {- dot -} UIO {- dot -} NO
Date: 2012-03-07


Subject: Re: FW: endotoxin removal
From: Kevin Jin kevinjin {- at -} GMAIL {- dot -} COM
Date: 2012-03-07

I tried to use 250mM Tris pH 7.6, 1M NaCl and 5~10% 2-propanol for
dialysis. It works,



On Wed, Mar 7, 2012 at 6:50 AM, Jerry McCully
wrote:
>
>
> Dear All;
>
>         We purified a His tag protein by Ni-NTA and gel-filtration from
> E.coli.
>
>          We tried two endotoxin removal resins from Pierce. However, it is
> hard to remove the endotoxins in the purified protein because the protein
> bound to the resin as well.
>
>          This protein contains quite a few aromatic residues and has a pI
> around 6.
>
>          Any ideas to remove the endotoxins will be highly appreciated.
>
>         best regards,
>
> Jerry McCully
>
>




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