Re: [ccp4bb] bfactor, freeR problems!!

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From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-10-01

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Subject: Re: bfactor, freeR problems!!
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-10-01

Yes to keeping the same FreeR
Bfactors - I doubt if it matters, but I usually start a new data set by
setting Bs to the Wilson suggestion..

Twinned data - maybe you ought to use SHELXL to refine it?

Eleanor

john kryst wrote:
> Hi all !!
>
> I am working on some deletion mutants. Mutants crystallized in
> the same space group with similar cell dimensions. My questions are the
> following??
>
> 1. Is the free R flag has to be maintained across all the mutants with
> respect to the native.
> 2. Does B-factor has to be flattened (say 30) before starting the
> refinement of the each mutant.
> 3. One of my mutant is twinned. should i maintain the same free R flag
> without worrying about the twinning ???
>
> Thanks for your inputs.
>
> with warm regards
> John
>
>

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CCP4bb <-- 2007 <-- October 2007 <-- 01 October 2007

Previous message:
Subject: Re: FreeR flag value swap
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-10-01

Next message:
Subject: Import and merge data collected from TWO sources?
From: Brenda Patterson ucbccka {- at -} UCL {- dot -} AC {- dot -} UK
Date: 2007-10-01