[ccp4bb] Zn fingers and Ni columns

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From: Raja Dey deyraja1 {- at -} YAHOO {- dot -} CO {- dot -} IN
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Subject: Re: Zn fingers and Ni columns
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-01-03

Subject: Zn fingers and Ni columns
From: price {- at -} UCHICAGO {- dot -} EDU price {- at -} UCHICAGO {- dot -} EDU
Date: 2008-01-03

This has probably been discussed before, so apologies in advance.
We're eyeing a protein that has a probable C4 Zn finger in the
middle. The collaborators who are nicely going to PCR it up want to
know if we'd like it with or without a His tag.
Is it a bad idea to co-mingle Zn-binders and Ni columns? Or is it
likely to bind the column quite nicely without the tag?
thanks,
Phoebe

---------------------------------------------------------------------------------------------------------------------------
Phoebe A. Rice
Assoc. Prof., Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
fax 773 702 0439
http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
http://www.nasa.gov/mission_pages/cassini/multimedia/pia06064.html

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CCP4bb <-- 2008 <-- January 2008 <-- 03 January 2008

Previous message:
Subject: Refmac 5.2.0019 failed in macbook
From: Raja Dey deyraja1 {- at -} YAHOO {- dot -} CO {- dot -} IN
Date: 2008-01-03

Next message:
Subject: Re: Zn fingers and Ni columns
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-01-03