[ccp4bb] How to refine large moleculars?

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CCP4bb <-- 2008 <-- January 2008 <-- 07 January 2008

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Subject: FW: alignment
From: Heidi Schubert heidi {- at -} BIOCHEM {- dot -} UTAH {- dot -} EDU
Date: 2008-01-07

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Subject: complement on how to refine large moleculars.
From: Yongchao Li yond_lee {- at -} YAHOO {- dot -} COM
Date: 2008-01-07

Subject: How to refine large moleculars?
From: Yongchao Li yond_lee {- at -} YAHOO {- dot -} COM
Date: 2008-01-07

I have a large molecule to refine.There are about three thousand residues in one asymmetric unit.
Except for the lack of electron densities in several places, where the
loops have been deleted, the rest parts fits well with density. But the R free and R work are all beyond 0.30 (R free = 0.35, R work = 0.30).
I don't know how to reduce them.
Can anyone give me some ideas?

Thank you!

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CCP4bb navigation

CCP4bb <-- 2008 <-- January 2008 <-- 07 January 2008

Previous message:
Subject: FW: alignment
From: Heidi Schubert heidi {- at -} BIOCHEM {- dot -} UTAH {- dot -} EDU
Date: 2008-01-07

Next message:
Subject: complement on how to refine large moleculars.
From: Yongchao Li yond_lee {- at -} YAHOO {- dot -} COM
Date: 2008-01-07