Re: [ccp4bb] resolution & R-free

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CCP4bb <-- 2008 <-- January 2008 <-- 08 January 2008

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Subject: how to model glycosylated residues?
From: Max Crispin max {- at -} STRUBI {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-01-08

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From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2008-01-08

Subject: Re: resolution & R-free
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-01-08

I doubt if you should ever t hrow away data if the I/SigI is sensible in
the outer shell.

Have you tried TLS refinement or loosened the B restraints? Often higher
Rfree at higher resolution are related to B refinement problems.
Eleanor

ÑîÁøÇà wrote:
> Hi,dear all:
> I try to refine one protein structure using refmac5,now I got some problems.
> For example,first,the resolution is 2.25,R-free is 30,completeness is 90%.
> Then I changed resolution to 2.35,R-free became 28.0,completeness is 91%.
> When I changed resolution to 2.45,R-free became 27.5,completeness is 92%.
> So,when resolution from 2.25 to 2.35,R-free goes down a lot,but resolution from 2.35 to 2.45,R-free nearly the same,is that meaning resolution should around 2.35? Should I change resolution during refinement? And is there any good software for refinement?
> Thanks a lot!
> Best wishes!
> liuqing
>
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CCP4bb <-- 2008 <-- January 2008 <-- 08 January 2008

Previous message:
Subject: how to model glycosylated residues?
From: Max Crispin max {- at -} STRUBI {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-01-08

Next message:
Subject: Re: how to model glycosylated residues?
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2008-01-08