Re: [ccp4bb] How to refine large moleculars?

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Subject: Re: Refmac 5.4.0066 versus Refmac 5.4.0034
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-01-08

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Subject: Re: how to model glycosylated residues?
From: junfeng liu jliu {- at -} NIMR {- dot -} MRC {- dot -} AC {- dot -} UK
Date: 2008-01-08

Subject: Re: How to refine large moleculars?
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-01-08

Yongchao Li wrote:
> I have a large molecule to refine.There are about three thousand residues in one asymmetric unit.
> Except for the lack of electron densities in several places, where the
> loops have been deleted, the rest parts fits well with density. But the R free and R work are all beyond 0.30 (R free = 0.35, R work = 0.30).
> I don't know how to reduce them.
> Can anyone give me some ideas?
>
> Thank you!
>
>
>
> ---------------------------------
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Size alone is not a problem.. think of viruses
But it is easy to make errors of course, and more time consuming to
check in such a long chain
what is the resolution though..

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CCP4bb <-- 2008 <-- January 2008 <-- 08 January 2008

Previous message:
Subject: Re: Refmac 5.4.0066 versus Refmac 5.4.0034
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-01-08

Next message:
Subject: Re: how to model glycosylated residues?
From: junfeng liu jliu {- at -} NIMR {- dot -} MRC {- dot -} AC {- dot -} UK
Date: 2008-01-08