[ccp4bb] Malic Acid interferes heavy metal binding?

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CCP4bb <-- 2008 <-- January 2008 <-- 09 January 2008

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Subject: Sulfate ion on 2-fold axis
From: Jie Liu jie {- at -} TRIMER {- dot -} MED {- dot -} CORNELL {- dot -} EDU
Date: 2008-01-09

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Subject: Re: Malic Acid interferes heavy metal binding?
From: William Scott wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-01-09

Subject: Malic Acid interferes heavy metal binding?
From: JINJIN ZHANG zhang {- dot -} 508 {- at -} OSU {- dot -} EDU
Date: 2008-01-09

Dear All,

I have got large and nice crystals in a condition that contains 40% glycerol, 75 mM DL-Malic Acid pH7.0, and that's it! No precipitant?! No buffer?! I'm so comfused. As far as from the product website, the Malic Acid has no buffering function. I don't know whether it's a precipitant. The protein itself was stored in a buffer contains 20mM Tris pH7.5, 150mM NaCl and 10% glycerol. I'm having difficulties in getting heavy metal bound derivatives. I was wondering whether the Malic Acid interferes heavy metal binding? Does anyone has any success with crystals grow with similar conditions? Any comment and suggestion is highly appreciated.

Regards,

Jinjin Zhang

CCP4bb navigation

CCP4bb <-- 2008 <-- January 2008 <-- 09 January 2008

Previous message:
Subject: Sulfate ion on 2-fold axis
From: Jie Liu jie {- at -} TRIMER {- dot -} MED {- dot -} CORNELL {- dot -} EDU
Date: 2008-01-09

Next message:
Subject: Re: Malic Acid interferes heavy metal binding?
From: William Scott wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-01-09