[ccp4bb] monomer library in refmac5

Protein crystallography Programs for crystallography X-ray detectors
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CCP4bb <-- 2007 <-- February 2007 <-- 28 February 2007

Previous message:
Subject: Re: Cannot running NTA to purify the protein having His-tag?
From: sabrina {- dot -} biarrotte-sorin {- at -} MAIL {- dot -} MCGILL {- dot -} CA
Date: 2007-02-28

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Subject: Re: Cannot running NTA to purify the protein having His-tag?
From: jmdias {- at -} SCRIPPS {- dot -} EDU
Date: 2007-02-28

Subject: monomer library in refmac5
From: zhu5 {- at -} PURDUE {- dot -} EDU
Date: 2007-02-28

Dear all,

I have some N-acetyl-glucosamine (NAG) in my structure. When I
searched the monomer library in CCP4 6.0.1, I found out that NAG is
actually N-acetyl-glucose, which is much less common, I believe. I
downloaded the high-resolution structure of N-acetyl-glucosamine from
HIC-UP and used sketcher to generate a cif library. I named the
sugar NAG. I read this new cif file into refmac5 (5.3) and used
"make check none" in the refinement. I thought my library would
overwrite the NAG in the monomer library in CCP4, but refmac5 still
use the NAG it found from the monomer library. Is there anyway to
make sure refmac5 will use my library even there is another one in
the monomer library with the same name?

Thanks.

Jianghai

CCP4bb navigation

CCP4bb <-- 2007 <-- February 2007 <-- 28 February 2007

Previous message:
Subject: Re: Cannot running NTA to purify the protein having His-tag?
From: sabrina {- dot -} biarrotte-sorin {- at -} MAIL {- dot -} MCGILL {- dot -} CA
Date: 2007-02-28

Next message:
Subject: Re: Cannot running NTA to purify the protein having His-tag?
From: jmdias {- at -} SCRIPPS {- dot -} EDU
Date: 2007-02-28