Re: [ccp4bb] Malic Acid interferes heavy metal binding?

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Subject: Re: Malic Acid interferes heavy metal binding?
From: Jim Pflugrath jim {- dot -} pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2008-01-14

Did you grow Malic Acid crystals?

I see no reason why glycerol cannot be the precipitant. I am aware that
at least one protein is crystallized with ethylene glycol as the
precipitant.

On Wed, 9 Jan 2008, JINJIN ZHANG wrote:

>
> Dear All,
>
> I have got large and nice crystals in a condition that contains 40%
> glycerol, 75 mM DL-Malic Acid pH7.0, and that's it! No precipitant?! No
> buffer?! I'm so comfused. As far as from the product website, the Malic
> Acid has no buffering function. I don't know whether it's a precipitant.
> The protein itself was stored in a buffer contains 20mM Tris pH7.5, 150mM
> NaCl and 10% glycerol. I'm having difficulties in getting heavy metal
> bound derivatives. I was wondering whether the Malic Acid interferes
> heavy metal binding? Does anyone has any success with crystals grow with
> similar conditions? Any comment and suggestion is highly appreciated.
>
> Regards,
>
> Jinjin Zhang

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CCP4bb <-- 2008 <-- January 2008 <-- 14 January 2008

Previous message:
Subject: Re: crystallisation robot
From: Alexey Rak Alexey {- dot -} Rak {- at -} SANOFI-AVENTIS {- dot -} COM
Date: 2008-01-14

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Subject: Re: bond lengths, angles, ideality and refinements
From: Morten Kjeldgaard mok {- at -} BIOXRAY {- dot -} AU {- dot -} DK
Date: 2008-01-14