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Re: [ccp4bb] crystallisation robot |
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CCP4bb navigationCCP4bb <-- 2008 <-- January 2008 <-- 16 January 2008Subject: Re: crystallisation robot From: Shirley Roberts roberts {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK Date: 2008-01-16 If I'm optimising in 24 well trays after a hit in 96 I generally use sitting drop trays (cryschem) as a first step and try different ratios of protein:mother liquor first rather than changing the protein concentration. I know that sometimes the crystals stick to the plastic but I use the hypodermic trick mentioned on the BB a few days ago. On the subject of drop size - I was at the RAMC meeting where this was discussed and several attendees had looked at the issue, 300+300nl or 400+400nl were mentioned as more optimal drop sizes for obtaining crystal hits during screening.See summary on: http://www.hamptonresearch.com/stuff/RAMC/RAMC2007Notes.aspx At York we use 150+150nl routinely for screening using the mosquito. I've moved up to 200+200 since the conference! Shirley Roberts ps I think I was responsible for the z to s change in the title.... CCP4bb navigationCCP4bb <-- 2008 <-- January 2008 <-- 16 January 2008 |
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