[ccp4bb] How to improve the density of another molecule in Asymmetric unit

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Subject: How to improve the density of another molecule in Asymmetric unit
From: Sun Tang suntang2222 {- at -} YAHOO {- dot -} COM
Date: 2008-01-16

Hello Everyone,

I have a question about how to improve the electron density. I have two indepedent molecules in the asymmetric unit (1.9 A resolution). I solved the structure with PHASER and refined with CCP4i (Rfree = 0.29).

For the first molecule, the density is very good, but for the second one, the density is much worse than the first one. Are there any ways to improve the density of the second molecule, such as some kinds of averaging? Why that happens?

Thank you very much for your help!

Sincerely,

Sun Tang
suntang2222@yahoo.com

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CCP4bb navigation

CCP4bb <-- 2008 <-- January 2008 <-- 16 January 2008

Previous message:
Subject: Re: crystallisation robot
From: Lisa A Nagy lisanagy {- at -} UAB {- dot -} EDU
Date: 2008-01-16

Next message:
Subject: postdoc position in structural biology of wound healing
From: David Hulmes d {- dot -} hulmes {- at -} IBCP {- dot -} FR
Date: 2008-01-16