Quick navigation:        Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |   
Protein structure
 

Re: [ccp4bb] Potassium Nitrate

 

Basic tutorials:
 
 

CCP4bb navigation

CCP4bb <-- 2008 <-- January 2008 <-- 16 January 2008
Previous message:
Subject: Potassium Nitrate
From: sajid akthar b_sajid_ali {- at -} YAHOO {- dot -} CO {- dot -} IN
Date: 2008-01-16
Next message:
Subject: Off topic, but am desparate...
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-01-16


Subject: Re: Potassium Nitrate
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2008-01-16

sajid akthar wrote:
>
> Hi All
>
>
>
> Sorry for non CCP4 question. I want to make Pottasium Nitrate solution
> of pH 6.9 (ofcourse for crystallisation trails only). I'm adjusting pH
> by using NaOH. But it seems the pH is not stable after sometime. Is
> there any good way to do it.
>
>
>
> Thank you
>
>
>
> Sajid
>
>
> ------------------------------------------------------------------------
> Now you can chat without downloading messenger. Click here
>
> to know how.
I would think a solution potassium nitrate in deionized water would have
a negligible effect on the pH of a buffered crystallization solution. I
wouldn't bother trying to adjust the pH, because the solution is not
sufficiently buffered, and depending on the ionic strength of the
solution, may not have sufficient conductivity to allow a pH electrode
to work properly.

Cheers,


--
------------------------------------------------------------------------
Roger S. Rowlett
Professor
Colgate University Presidential Scholar
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346

tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: rrowlett@mail.colgate.edu

CCP4bb navigation

CCP4bb <-- 2008 <-- January 2008 <-- 16 January 2008
Previous message:
Subject: Potassium Nitrate
From: sajid akthar b_sajid_ali {- at -} YAHOO {- dot -} CO {- dot -} IN
Date: 2008-01-16
Next message:
Subject: Off topic, but am desparate...
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-01-16



ProteinCrystallography.org: Copyright 2006-2007 by Quid United Ltd