[ccp4bb] improve crystal size and quality -- membrane protein

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CCP4bb <-- 2007 <-- February 2007 <-- 28 February 2007

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Subject: Re: homology modeling----good bond lengths, bad angles
From: sudhaarsan {- at -} GMAIL {- dot -} COM
Date: 2007-02-28

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Subject: Re: Cannot running NTA to purify the protein having His-tag?
From: artem {- at -} XTALS {- dot -} ORG
Date: 2007-02-28

Subject: improve crystal size and quality -- membrane protein
From: Balaji {- dot -} Bhyravbhatla {- at -} UMASSMED {- dot -} EDU
Date: 2007-02-28

Hello All,
We are trying to crystallize a membrane protein but cannot get the xtals to grow bigger. Presently we have only thin needles (diffracting to about 8A). Thus far we have tried to change protein concentration, LDAO concentration, PEG screen as well as temperature. Have tried macro and micro seeding as well.

A typical setup looks like: 10% PEG 6000, 0.1M Citrate 5.5, 0.1M Li2SO4 and 5% MPD with about 12mgs/ml of protein which has about 0.2% LDAO

Any suggestions or improvements that we can try would be appreciated. This construct of the protein is not in the membrane but membrane associated.

Thanks

Balaji

CCP4bb navigation

CCP4bb <-- 2007 <-- February 2007 <-- 28 February 2007

Previous message:
Subject: Re: homology modeling----good bond lengths, bad angles
From: sudhaarsan {- at -} GMAIL {- dot -} COM
Date: 2007-02-28

Next message:
Subject: Re: Cannot running NTA to purify the protein having His-tag?
From: artem {- at -} XTALS {- dot -} ORG
Date: 2007-02-28