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Re: [ccp4bb] Codon Optimized Expression |
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CCP4bb navigationCCP4bb <-- 2008 <-- February 2008 <-- 02 February 2008Subject: Re: Codon Optimized Expression From: price {- at -} UCHICAGO {- dot -} EDU price {- at -} UCHICAGO {- dot -} EDU Date: 2008-02-02 near the beginning, can be extra-bad. For one small, A/T rich protein, we simply "fixed" a pair of bad Arg codons near the N-terminus at the same time as recloning it, by using the N-terminal cloning primer to do the mutagenesis. Combined with using codon-plus E coli, expression improved dramatically. Good luck! Phoebe Rice At 09:29 AM 2/1/2008, Looney, Andrea Lynn (Andrea Hevrdeys) wrote: >Dear All, > >I am getting very little (not zero) expression of my protein. I am >curious to know if it is worthwhile to codon optimize my gene, which >is ~1200bp, for E.coli. Can you have "too much of a good >thing"? Can codon optimizing overwhelm the cell or in some way >cause death or lowered expression? > >Thank you all, > >Andrea L. Hevrdeys > --------------------------------------------------------------------------------------------------------------------------- Phoebe A. Rice Assoc. Prof., Dept. of Biochemistry & Molecular Biology The University of Chicago phone 773 834 1723 fax 773 702 0439 http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 http://www.nasa.gov/mission_pages/cassini/multimedia/pia06064.html CCP4bb navigationCCP4bb <-- 2008 <-- February 2008 <-- 02 February 2008 |
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