[ccp4bb] AW: [ccp4bb] Thermofluor

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From: Dale Tronrud det102 {- at -} UOXRAY {- dot -} UOREGON {- dot -} EDU
Date: 2008-02-12

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Subject: AW: Thermofluor
From: Ralf JAUCH jauchr {- at -} GIS {- dot -} A-STAR {- dot -} EDU {- dot -} SG
Date: 2008-02-12

Hi Jeroen,

I assume you are planning to use Sypro Orange. We are using the Roche LC480 (simply because it's around in the lab next door) and it does the job although it's a little inconvenient to tweak the settings.

Many people use the iCycler from Biorad. This machine is a little more flexible and can be customized: You can introduce filter pairs of your choice and play with other dyes such as ANS-1.8. We intend to do that since sypro orange binds to quite a number of our proteins before heating (which perhaps tells you something about our proteins but lets blame the dye first).

Ralf

Ralf Jauch
Genome Institute of Singapore
60 Biopolis Street
#02-01Genome
Singapore 138672
Tel: (65) 6478 8102
Mobile: (65) 96398715
jauchr@gis.a-star.edu.sg

-----Ursprüngliche Nachricht-----
Von: CCP4 bulletin board im Auftrag von mesters
Gesendet: Mi 13.02.2008 01:07
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] Thermofluor

Sorry for the off-topic but can somebody recommend highly a sensitive
RT-PCR machine for the thermofluor experiment (sypro orange).
That would imply excitation below 500 nm (ideally 470) and detection at
about 570 nm, right? I know several simple machines have a problem with
the 570 nm...

Jeroen.

--
Jeroen Raymundus Mesters, Ph.D.
Institut fuer Biochemie, Universitaet zu Luebeck
Zentrum fuer Medizinische Struktur und Zellbiologie
Ratzeburger Allee 160, D-23538 Luebeck
Tel: +49-451-5004070, Fax: +49-451-5004068
E-mail: mesters@biochem.uni-luebeck.de
Http://www.biochem.uni-luebeck.de
Http://www.iobcr.org
Http://www.opticryst.org
--
If you can look into the seeds of time and say
which grain will grow and which will not - speak then to me (Macbeth)
--

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CCP4bb <-- 2008 <-- February 2008 <-- 12 February 2008

Previous message:
Subject: Re: an over refined structure
From: Dale Tronrud det102 {- at -} UOXRAY {- dot -} UOREGON {- dot -} EDU
Date: 2008-02-12

Next message:
Subject: Re: Fit2D?
From: Alexander Johs ccp4 {- at -} GMX {- dot -} NET
Date: 2008-02-12