Re: [ccp4bb] Thermofluor

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Subject: Re: summary: sgi dials on intel mac
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-02-13

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Subject: Thermofluor experiment
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2008-02-13

Subject: Re: Thermofluor
From: Jendrek alnyds {- at -} GMAIL {- dot -} COM
Date: 2008-02-13

Hei Jeroen,

Quite recently we got BioRad MiniOpticon system for the thermoflor based
assays. I think this is the least expensive RT-PCR on the market but it performs
very well in our experiments.

It can excite the samples between 470 and 505 nm and one of the detection
channel covers the Sypro Orange emission.
The control software is VERY easy to use.

Andrzej, HY

mesters wrote:
> Sorry for the off-topic but can somebody recommend highly a sensitive
> RT-PCR machine for the thermofluor experiment (sypro orange).
> That would imply excitation below 500 nm (ideally 470) and detection at
> about 570 nm, right? I know several simple machines have a problem with
> the 570 nm...
>
> Jeroen.
>

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CCP4bb <-- 2008 <-- February 2008 <-- 13 February 2008

Previous message:
Subject: Re: summary: sgi dials on intel mac
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-02-13

Next message:
Subject: Thermofluor experiment
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2008-02-13