[ccp4bb] Thermofluor experiment

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Subject: Re: Thermofluor
From: Jendrek alnyds {- at -} GMAIL {- dot -} COM
Date: 2008-02-13

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Subject: Re: Thermofluor experiment
From: mesters mesters {- at -} BIOCHEM {- dot -} UNI-LUEBECK {- dot -} DE
Date: 2008-02-13

Subject: Thermofluor experiment
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2008-02-13

Dear all,

a question very related to the discussion before. I have been reading the papers about the thermofluor experiment with great interest.

I wonder what people think about the underlying principles/ideas and the success that the method yielded in their own labs for crystallization or related purposes?

Has anybody used this method with membrane proteins in order to find out the stability of the protein in the presence of a second detergent?

Is the method limited to this certain dye (sypro orange)?

Have a nice day

Kornelius

P.S.: I will make a summary of all opinions.

----------------------------------------------
Kornelius Zeth
Max Planck Institute for Developmental Biology
Dept. Protein Evolution
Spemannstr. 35
72076 Tuebingen, Germany
kornelius.zeth@tuebingen.mpg.de
Tel -49 7071 601 323
Fax -49 7071 601 349

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CCP4bb <-- 2008 <-- February 2008 <-- 13 February 2008

Previous message:
Subject: Re: Thermofluor
From: Jendrek alnyds {- at -} GMAIL {- dot -} COM
Date: 2008-02-13

Next message:
Subject: Re: Thermofluor experiment
From: mesters mesters {- at -} BIOCHEM {- dot -} UNI-LUEBECK {- dot -} DE
Date: 2008-02-13