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Re: [ccp4bb] Thermofluor experiment
 

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Subject: Thermofluor experiment
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2008-02-13 		Next message:
Subject: Re: xprep vs shelxc
From: Bram Schierbeek bram {- dot -} schierbeek {- at -} BRUKER-AXS {- dot -} NL
Date: 2008-02-13


Subject: Re: Thermofluor experiment
From: mesters mesters {- at -} BIOCHEM {- dot -} UNI-LUEBECK {- dot -} DE
Date: 2008-02-13
Kornelius,

have a look at the following paper:

Yeh AP, McMillan A, Stowell MH.



Rapid and simple protein-stability screens: application to membrane
proteins.
Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):451-7. Epub 2006
Mar 18.

- J. -



Kornelius Zeth wrote:
> Dear all,
>
> a question very related to the discussion before. I have been reading the papers about the thermofluor experiment with great interest.
>
> I wonder what people think about the underlying principles/ideas and the success that the method yielded in their own labs for crystallization or related purposes?
>
> Has anybody used this method with membrane proteins in order to find out the stability of the protein in the presence of a second detergent?
>
> Is the method limited to this certain dye (sypro orange)?
>
> Have a nice day
>
> Kornelius
>
> P.S.: I will make a summary of all opinions.
>
> ----------------------------------------------
> Kornelius Zeth
> Max Planck Institute for Developmental Biology
> Dept. Protein Evolution
> Spemannstr. 35
> 72076 Tuebingen, Germany
> kornelius.zeth@tuebingen.mpg.de
> Tel -49 7071 601 323
> Fax -49 7071 601 349
>


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Jeroen Raymundus Mesters, Ph.D.
Institut fuer Biochemie, Universitaet zu Luebeck
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