Re: [ccp4bb] Thermofluor

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CCP4bb <-- 2008 <-- February 2008 <-- 13 February 2008

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Subject: Re: xprep vs shelxc
From: Bram Schierbeek bram {- dot -} schierbeek {- at -} BRUKER-AXS {- dot -} NL
Date: 2008-02-13

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Subject: Re: xprep vs shelxc
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-02-13

Subject: Re: Thermofluor
From: mesters mesters {- at -} BIOCHEM {- dot -} UNI-LUEBECK {- dot -} DE
Date: 2008-02-13

yes indeed, the Biorad MiniOpticon is the cheapest so far. Next one on
my list is the Applied Biosystems StepOne. Can anybody comment on the
latter machine (not the more expensive StepOnePlus)?

The machines proposed like Roche LC480, Stratagene Mx3005p or Biorad iQ5
are far beyond 30 k€ list price..... beyond my budget.....

Not only do you need to have a close look at the available detection
filters and their bandwidth (latter can be very different from company
to company!) but also the software is very important because we do the
opposite of a real PCR experiment, that is, curves are turned around and
many software packages can not handle that or, even worse stop
working... In this case, you need to be able to export the data in a
usable format!

- J. -

Jendrek wrote:
> Hei Jeroen,
>
> Quite recently we got BioRad MiniOpticon system for the thermoflor
> based assays. I think this is the least expensive RT-PCR on the market
> but it performs very well in our experiments.
>
> It can excite the samples between 470 and 505 nm and one of the
> detection channel covers the Sypro Orange emission.
> The control software is VERY easy to use.
>
> Andrzej, HY
>
> mesters wrote:
>> Sorry for the off-topic but can somebody recommend highly a sensitive
>> RT-PCR machine for the thermofluor experiment (sypro orange).
>> That would imply excitation below 500 nm (ideally 470) and detection
>> at about 570 nm, right? I know several simple machines have a problem
>> with the 570 nm...
>>
>> Jeroen.
>>

--
Jeroen Raymundus Mesters, Ph.D.
Institut fuer Biochemie, Universitaet zu Luebeck
Zentrum fuer Medizinische Struktur und Zellbiologie
Ratzeburger Allee 160, D-23538 Luebeck
Tel: +49-451-5004070, Fax: +49-451-5004068
E-mail: mesters@biochem.uni-luebeck.de
Http://www.biochem.uni-luebeck.de
Http://www.iobcr.org
Http://www.opticryst.org
--
If you can look into the seeds of time and say
which grain will grow and which will not - speak then to me (Macbeth)
--

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CCP4bb <-- 2008 <-- February 2008 <-- 13 February 2008

Previous message:
Subject: Re: xprep vs shelxc
From: Bram Schierbeek bram {- dot -} schierbeek {- at -} BRUKER-AXS {- dot -} NL
Date: 2008-02-13

Next message:
Subject: Re: xprep vs shelxc
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-02-13