Re: [ccp4bb] Anti-6His antibody

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 13 February 2008

Previous message:
Subject: Anti-6His antibody
From: Hongmin Li lih {- at -} WADSWORTH {- dot -} ORG
Date: 2008-02-13

Next message:
Subject: open position: Support Scientist Macromolecular Crystallography at Diamond Light Source
From: "Flaig, R (Ralf)" ralf {- dot -} flaig {- at -} DIAMOND {- dot -} AC {- dot -} UK
Date: 2008-02-13

Subject: Re: Anti-6His antibody
From: hua jing jing_hua {- at -} HOTMAIL {- dot -} COM
Date: 2008-02-13

Hi Hongmin,

I have used the anti-penta-His HRP conjugate antibody from Qiagen, and it works very well. It comes as a kit which includes a special blocking buffer. You don't need to use any secondary antibody, so it saves time. But you can only use the diluted solution 1-2 times within 1-2 weeks, so it is not as re-usable as most primary antibodies. See details at Qiagen web site.
http://www1.qiagen.com/Products/Protein/Detection/QIAexpressDetectionSystem/Penta-HisHRPConjugateKit.aspx

My friend also had good experience with a cheaper anti-His antibody from Sigma. But it didn't work well in our hands probably because it prefers N-terminal His tag.
http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/H1029

HTH.

Hua
PS: I am at Scripps now and Lei is at UCLA.

----------------------------------------
> Date: Wed, 13 Feb 2008 11:54:05 -0500
> From: lih@WADSWORTH.ORG
> Subject: [ccp4bb] Anti-6His antibody
> To: CCP4BB@JISCMAIL.AC.UK
>
> Hi,
>
> We are looking for a good and price-effective anti-6His antibody for
> detection of protein expressions (western or ELISA). Anyone can give
> some suggestions (brand, cat no, etc)? Thank you very much.
>
> Hongmin Li, PhD
> Wadsworth Center
> New York State Department of Health
>
>
>
>
> IMPORTANT NOTICE: This e-mail and any attachments may contain
> confidential or sensitive information which is, or may be, legally
> privileged or otherwise protected by law from further disclosure. It
> is intended only for the addressee. If you received this in error or
> from someone who was not authorized to send it to you, please do not
> distribute, copy or use it or any attachments. Please notify the
> sender immediately by reply e-mail and delete this from your
> system. Thank you for your cooperation.

_________________________________________________________________
Connect and share in new ways with Windows Live.
http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012008

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 13 February 2008

Previous message:
Subject: Anti-6His antibody
From: Hongmin Li lih {- at -} WADSWORTH {- dot -} ORG
Date: 2008-02-13