[ccp4bb] secondary structure restraints

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 13 February 2008

Previous message:
Subject: Re: Thermofluor experiment
From: "Das, Debanu" debanu {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2008-02-13

Next message:
Subject: Re: secondary structure restraints
From: Raji Edayathumangalam redayath {- at -} MAIL {- dot -} ROCKEFELLER {- dot -} EDU
Date: 2008-02-13

Subject: secondary structure restraints
From: Sean Johnson sean {- at -} CC {- dot -} USU {- dot -} EDU
Date: 2008-02-13

I am trying to build and refine a model into 3.6 angstrom Se-met phased
maps. What is the best way to define secondary structure restraints for
refinement? (hydrogen bonds? backbone torsion angles?) Are there any
tools available to help me define restraints for a specified region, or
do I have to define each restraint one at a time (which strikes me as a
very tedious exercise).

Any other thoughts on best practices for low-resolution model building
and refinement would also be appreciated.

Thanks,
Sean

--
Sean Johnson, PhD
R. Gaurth Hansen Assistant Professor
Utah State University
Department of Chemistry and Biochemistry
0300 Old Main Hill
Logan, UT 84322-0300
(435) 797-2089
(435) 797-3390 (fax)
sean.johnson@usu.edu

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 13 February 2008

Previous message:
Subject: Re: Thermofluor experiment
From: "Das, Debanu" debanu {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2008-02-13

Next message:
Subject: Re: secondary structure restraints
From: Raji Edayathumangalam redayath {- at -} MAIL {- dot -} ROCKEFELLER {- dot -} EDU
Date: 2008-02-13