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Re: [ccp4bb] crashing-out protein eluted from Nickel column |
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CCP4bb navigationCCP4bb <-- 2008 <-- February 2008 <-- 15 February 2008Subject: Re: crashing-out protein eluted from Nickel column From: Antony Oliver Antony {- dot -} Oliver {- at -} ICR {- dot -} AC {- dot -} UK Date: 2008-02-15 Dr Antony W Oliver Cancer Research UK: DNA Repair Enzymes Group Section of Structural Biology The Institute of Cancer Research 237 Fulham Road LONDON SW3 6JB antony.oliver@icr.ac.uk 020 7153 5488 -- >>> Juergen Bosch Not sure if somebody mentioned it already, but have you played with other buffers than the recommended sodium phosphate from the manual ? Most lazy people use Tris because there's a 1 M stock solution - but you can try also other buffers as long as you stay in a pH range >pH6 bring some surprises, one of them can be a better initial purity after the first capture step, another one might stabilize your protein. Juergen -- Jürgen Bosch University of Washington Dept. of Biochemistry, K-426 1705 NE Pacific Street Seattle, WA 98195 Box 357742 Phone: +1-206-616-4510 FAX: +1-206-685-7002 Web: http://faculty.washington.edu/jbosch The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP. This e-mail message is confidential and for use by the addressee only. If the message is received by anyone other than the addressee, please return the message to the sender by replying to it and then delete the message from your computer and network. CCP4bb navigationCCP4bb <-- 2008 <-- February 2008 <-- 15 February 2008 |
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