Re: [ccp4bb] Heavy atom soaking

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Subject: Re: crashing-out protein eluted from Nickel column
From: Antony Oliver Antony {- dot -} Oliver {- at -} ICR {- dot -} AC {- dot -} UK
Date: 2008-02-15

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Subject: Re: rescuing crashing-out protein eluted from Nickel column
From: Jacob Wong jacob {- dot -} j {- dot -} wong {- at -} GMAIL {- dot -} COM
Date: 2008-02-16

Subject: Re: Heavy atom soaking
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-02-15

Could you try a quick (10-15s) halide soak (Iodine) with e.g. 1M NaI?
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

GPG Key ID = A46BEE1A

On Fri, 15 Feb 2008, john peter wrote:

> Dear Folks,
> I recently grew crystals in the presence of 100 mM Imidazole pH 8.0 and 1
> M Ammonium Phosphate. The protein was in 25 mM Hepes pH 7.4, 10 mM EDTA, 10
> mM EGTA and 100 mM NaCl. I like to make heavy atom derivatives with Hg, Pt,
> Sm, Au and Pb compounds. I think the imidazole will interfere with Pt.
> EDTA and EGTA may also interfere with cations. However I'm not sure about
> the effect of phosphate. I tried to transfer the crystal to PEGs by
> gradually decreasing the AmPO4 concentration and increasing the PEG
> concentration, but the crystals deteriorate. I'm wondering what successful
> strategy people have employed to make good heavy atom derivatives when the
> mother liquor has salt as the major precipitant. All suggestions, tips are
> greatly appreciated. Thanks a lot.
>
> John
>

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CCP4bb <-- 2008 <-- February 2008 <-- 15 February 2008

Previous message:
Subject: Re: crashing-out protein eluted from Nickel column
From: Antony Oliver Antony {- dot -} Oliver {- at -} ICR {- dot -} AC {- dot -} UK
Date: 2008-02-15

Next message:
Subject: Re: rescuing crashing-out protein eluted from Nickel column
From: Jacob Wong jacob {- dot -} j {- dot -} wong {- at -} GMAIL {- dot -} COM
Date: 2008-02-16