Re: [ccp4bb] crashing-out protein eluted from Nickel column

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 18 February 2008

Previous message:
Subject: Re: XDS - resolution limit
From: Kay Diederichs Kay {- dot -} Diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2008-02-18

Next message:
Subject: indexing multiple lattices
From: Jason Greenwald jason {- dot -} greenwald {- at -} PHYS {- dot -} CHEM {- dot -} ETHZ {- dot -} CH
Date: 2008-02-18

Subject: Re: crashing-out protein eluted from Nickel column
From: Ngo Duc Tri ngoductri {- at -} GMAIL {- dot -} COM
Date: 2008-02-18

Hi,
I used another way to deal with this problem. You can try to elute your
protein with the buffer containing 50mM EDTA (You need at least 10CV to
elute completely). Then use gel filtration to remove the Ni.

I applied this method with two proteins and it showed good results.
Good luck!

TriNgo
----------------------
PhD student,
Sungkyunkwan University

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 18 February 2008

Previous message:
Subject: Re: XDS - resolution limit
From: Kay Diederichs Kay {- dot -} Diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2008-02-18

Next message:
Subject: indexing multiple lattices
From: Jason Greenwald jason {- dot -} greenwald {- at -} PHYS {- dot -} CHEM {- dot -} ETHZ {- dot -} CH
Date: 2008-02-18