[ccp4bb] Side chain chemical modifiers

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CCP4bb <-- 2008 <-- February 2008 <-- 21 February 2008

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Subject: Re: XDS and overlaps
From: Phil Evans pre {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-02-21

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Subject: Re: Side chain chemical modifiers
From: "Das, Debanu" debanu {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2008-02-21

Subject: Side chain chemical modifiers
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-02-21

Dear Crystallographers,

I am looking for a way to modify any specific set of sidechains in my
protein (e.g., all K's, or all D's and E's, etc.). A few requirements:

1. Relatively cheap
2. Definitely non-cell-membrane-crossing
3. Specific
4. Any reasonable amount of change in mass (+ 5-5000 Da?), charge, etc., is
fine
5. Preferably less life threatening to live cells (I know this is a probably
a losing battle)

The last criterion is because I want to modify the protein in vivo, then
immediately harvest the cells, etc. I was hoping there would be something
"around the house" that I could use for this, especially for hitting K's.

All the best,

Jacob

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-keller2@northwestern.edu
*******************************************

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 21 February 2008

Previous message:
Subject: Re: XDS and overlaps
From: Phil Evans pre {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-02-21

Next message:
Subject: Re: Side chain chemical modifiers
From: "Das, Debanu" debanu {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2008-02-21