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Re: [ccp4bb] unknown symmetric density
 

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CCP4bb <-- 2008 <-- February 2008 <-- 26 February 2008
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Subject: Re: unknown symmetric density
From: Partha Chakrabarti ppchak {- at -} GMAIL {- dot -} COM
Date: 2008-02-26 		Next message:
Subject: Meeting announcement: SGC Oxford Symposium - Structural Biology and Human Health, Monday April 14th 2008
From: Ashley Pike Ashley {- dot -} Pike {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-02-26


Subject: Re: unknown symmetric density
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2008-02-26
Oh......
Thank you. I will try to fit it with a piece of PEG.
Best wishes.

On Tue, Feb 26, 2008 at 10:38 PM, wrote:

> Hi Jiamu,
>
> This is almost surely a nic piece you PEG3350.
>
> Regards,
>
> zsolt
>
>
> Dr. Zsolt Bocskei
> sanofi-aventis
> Chemical Sciences Department
> 16 rue d'Ankara
> 67000 Strasbourg
> France
>
> ------------------------------
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *Jiamu
> Du
> *Sent:* Tuesday, February 26, 2008 3:33 PM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] unknown symmetric density
>
> Dear All,
> Here I am refining a 2.0 angstrom resolution structure of a protein in
> complex with a 18mer peptide. Now the refined model has a R/Rfree value of
> 0.206/0.266. But there is no obvious density for the peptide. An
> interesting observation is that some strange density is located in the
> proposed protein-peptide interaction interface as shown in the attachment.
> The shown map is SACO map at 1.0 sigma level. The strange density is near
> the 2-fold axis of the crystal cell, so the density is also 2-fold
> symmetric. Although I traced it with solvent molecules, I believe it is not.
> It seems not the peptide, either. But I can not image what it is. What do
> you think?
> The crystal condition contains Tris, Hepes, NaCl, ZnAc, and PEG3350.
> Thanks.
>
> --
> Jiamu Du
> State Key Laboratory of Molecular Biology
> Institute of Biochemistry and Cell Biology Shanghai Institutes for
> Biological Sciences
> Chinese Academy of Sciences (CAS)
>



--
Jiamu Du
State Key Laboratory of Molecular Biology
Institute of Biochemistry and Cell Biology Shanghai Institutes for
Biological Sciences
Chinese Academy of Sciences (CAS)
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