Re: [ccp4bb] Glu+Arg solutions for solubility

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Subject: Glu+Arg solutions for solubility
From: Paul Paukstelis paul {- at -} ICMB {- dot -} UTEXAS {- dot -} EDU
Date: 2008-02-28

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Subject: Off topic: General rule for maximum flow rate for affinity column?
From: Eric Dollins d {- dot -} e {- dot -} dollins {- at -} GMAIL {- dot -} COM
Date: 2008-02-28

Subject: Re: Glu+Arg solutions for solubility
From: "Jensen, Jan Kristian" jkj {- at -} UIC {- dot -} EDU
Date: 2008-02-28

I have not tried to make 1M solutions of the indicated AA, but i have had
good luck of using what they suggest in that specific paper. By using 50mM
of each (Glu and Arg) I was able to increase the solubility of a 44kDa
protein (a Serpin) from ~1mg/ml to above 17mg/ml. So in my case it really
worked. This was for the purpose of doing liquid state NMR on that
protein.
I does help to solubilize the two aa in 1:1 ratio as the two combined
improve their individual solubility.

Jan Jensen
On Thu, February 28, 2008 4:33 pm, Paul Paukstelis wrote:
> posting for a colleague:
>
> In Golovanov et al. 2004 they indicate it is possible to make a 1M
> solution of free amino acids (not amino acid salts) Glu+Arg in the
> context of generating solutions for keeping proteins soluble. Has anyone
> been able to do this?
>
>

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CCP4bb <-- 2008 <-- February 2008 <-- 28 February 2008

Previous message:
Subject: Glu+Arg solutions for solubility
From: Paul Paukstelis paul {- at -} ICMB {- dot -} UTEXAS {- dot -} EDU
Date: 2008-02-28

Next message:
Subject: Off topic: General rule for maximum flow rate for affinity column?
From: Eric Dollins d {- dot -} e {- dot -} dollins {- at -} GMAIL {- dot -} COM
Date: 2008-02-28