[ccp4bb] O-glycosylation in ccp4/refmac

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CCP4bb <-- 2008 <-- February 2008 <-- 28 February 2008

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Subject: Off topic: General rule for maximum flow rate for affinity column?
From: Eric Dollins d {- dot -} e {- dot -} dollins {- at -} GMAIL {- dot -} COM
Date: 2008-02-28

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Subject: Re: Off topic: General rule for maximum flow rate for affinity column?
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-02-28

Subject: O-glycosylation in ccp4/refmac
From: Rebecca Phillips rephilli {- at -} CHEM {- dot -} UCSD {- dot -} EDU
Date: 2008-02-28

Hello, I am trying to model an O-glycosylation modification at a
threonine, but this seems to be problematic when I try to refine the model
in refmac. The run fails, and the log says that a new ligand was found
(the sugar). It offers a lib.cif file to add to the library and try the
refinement again. I looked at this file and it looked fine (i.e. it wrote
out the correct bond that is made between the sugar and threonine).
However, when I tried to refine again with the lib.cif file, the run
failed again and gave the same message. Thanks for any advice!

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 28 February 2008

Previous message:
Subject: Off topic: General rule for maximum flow rate for affinity column?
From: Eric Dollins d {- dot -} e {- dot -} dollins {- at -} GMAIL {- dot -} COM
Date: 2008-02-28

Next message:
Subject: Re: Off topic: General rule for maximum flow rate for affinity column?
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-02-28