[ccp4bb] anomalous signal of Mn and Ca ions

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 28 February 2008

Previous message:
Subject: Re: Off topic: General rule for maximum flow rate for affinity column?
From: Stephen Weeks stephen {- dot -} weeks {- at -} VERIZON {- dot -} NET
Date: 2008-02-28

Next message:
Subject: Re: anomalous signal of Mn and Ca ions
From: Ethan A Merritt merritt {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-02-28

Subject: anomalous signal of Mn and Ca ions
From: Sun Tang suntang2222 {- at -} YAHOO {- dot -} COM
Date: 2008-02-28

Dear All,

In my structures, I want to assign Mn or Ca ions for some densities. But when I did not have anomalous density in CCP4i. I am not sure whether I was correct. The following was what I did:

I processed the data with HKL2000 and select anomalous signal in scaling. In CCP4i, I selected "Run FFT-Creat Map" in the "Map& Mask Utilities". I select "O format to cover asymmetric unit" and "Plot section on Z axis from 0 to 1 in steps on 10". All others were by default values. I display in ono10.

I collected the data at the wavelength of 1 A. Do I need to adjust the wavelength to maximize the anomalous signal from Mn or Ca?

Any ideas and suggestions are greatly appreciated!

Sun Tang

---------------------------------
Never miss a thing. Make Yahoo your homepage.

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 28 February 2008

Previous message:
Subject: Re: Off topic: General rule for maximum flow rate for affinity column?
From: Stephen Weeks stephen {- dot -} weeks {- at -} VERIZON {- dot -} NET
Date: 2008-02-28

Next message:
Subject: Re: anomalous signal of Mn and Ca ions
From: Ethan A Merritt merritt {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-02-28