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Re: [ccp4bb] Fwd: Re: [ccp4bb] Glu+Arg solutions for solubility
 

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CCP4bb <-- 2008 <-- March 2008 <-- 03 March 2008
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From: Brenda Patterson ucbccka {- at -} UCL {- dot -} AC {- dot -} UK
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Date: 2008-03-03


Subject: Re: Fwd: Re: Glu+Arg solutions for solubility
From: Stephen Prince steve {- dot -} prince {- at -} MANCHESTER {- dot -} AC {- dot -} UK
Date: 2008-03-03
from the horses mouth ..

----- Forwarded message from A.Golovanov@manchester.ac.uk -----
Date: Mon, 03 Mar 2008 10:16:41 +0000
From: Alexander Golovanov
Reply-To: Alexander Golovanov
Subject: Re: Fwd: Re: [ccp4bb] Glu+Arg solutions for solubility
To: Stephen Prince

Don't use salts, as it says (otherwise you will have to titrate pH a
lot to get it back to neutral). Just L-Arg and L-Glu. First, put
L-Arginine powder in some water, start dissolving it, then add L-Glu
powder, stir them together, top up the volume as required. They are
only soluble together at 1 M. The final pH of the 1M stock will be
(by itself) around 6.4 or close to neutral, in our experience.

good luck!
Sasha

At 09:17 03/03/2008, you wrote:

> ... from a bulletin board I subscribe to, are there any tricks for
> making the stock (pH?) ...
>
>
> On Thu, February 28, 2008 4:33 pm, Paul Paukstelis wrote:
>> posting for a colleague:
>>
>> In Golovanov et al. 2004 they indicate it is possible to make a 1M
>> solution of free amino acids (not amino acid salts) Glu+Arg in the
>> context of generating solutions for keeping proteins soluble. Has anyone
>> been able to do this?
>>
>
>
> ----- End forwarded message -----
>
>
> ______________________________________________________________________
>
> S.M. Prince Phone: +44 (0)161 306 8919
> Faculty of Life Sciences FAX: +44 (0)161 306 5201
> Manchester Interdisciplinary Biocentre
> University of Manchester
> 131 Princess Street e-mail: steve.prince@manchester.ac.uk
> Manchester
> M1 7DN
> U.K.
> ______________________________________________________________________

--
Dr Alexander 'Sasha' Golovanov
Lecturer
Faculty of Life Sciences and
Manchester Interdisciplinary Biocentre
The University of Manchester
131 Princess Street
Manchester M1 7DN, UK
Tel. No: +44 (0) 161 306 5813 Office (G.024)
+44 (0) 161 306 5812 NMR room
FAX: +44 (0) 161 306 5201
e-mail A.Golovanov@manchester.ac.uk
web: http://www.mib.manchester.ac.uk/aboutus/people/details.aspx?val=97941



----- End forwarded message -----


______________________________________________________________________

S.M. Prince Phone: +44 (0)161 306 8919
Faculty of Life Sciences FAX: +44 (0)161 306 5201
Manchester Interdisciplinary Biocentre
University of Manchester
131 Princess Street e-mail: steve.prince@manchester.ac.uk
Manchester
M1 7DN
U.K.
______________________________________________________________________
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