Re: [ccp4bb] Copper Staining

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 03 March 2008

Previous message:
Subject: Re: Copper Staining
From: Rebecca Phillips rephilli {- at -} CHEM {- dot -} UCSD {- dot -} EDU
Date: 2008-03-03

Next message:
Subject: Re: Copper Staining
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-03-03

Subject: Re: Copper Staining
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-03-03

There's an even faster reagent for staining - takes abut 1 minute overall
and the results look just like dear old Coomassie. This reagent works
wonderfully for us. Yes, the recipe is proprietary - but if you add up the
cost of reagents for normal Coomassie and compare - this one isn't much more
expensive.

http://www.novexin.com/products/InstantBlue.html

Artem

P.S. I am not affiliated with Novexin in any way shape or form, but their
gel stain does kick butt.

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob
Keller
Sent: Monday, March 03, 2008 6:03 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Copper Staining

Dear Crystallographers:

just to share something I found out recently, that is really helpful to
know:

5 min Copper Stain protocol

Normal SDS-PAGE gels can be stained in 5 min with 300mM CuCl2. Simply remove

the gel, rinse ~10s in water, place 5 min in CuCl2, and the gel is
negatively stained, with protein bands appearing clear, and background
opaque white. To visualize, I put it on a flatbed scanner in a darkened
room. It works in my hands every bit as well as coomassie (at least as
sensitive, if not more), and if you want, you can stain afterwards in
coomassie with the usual effect. To destain, place gel in EDTA. The original

paper is Lee et al, Anal. Biochem. 1987. Instead of waiting hours, you can
see the results in 5 min with copper! Also, there is no smell, and it can be

re-used, to a point. Gels are stable in H2O for months, say Lee et al.

Jacob

p.s. I am not in copper comodities...
p.p.s. if anybody knows of down-sides to this, please let me know.

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 03 March 2008

Previous message:
Subject: Re: Copper Staining
From: Rebecca Phillips rephilli {- at -} CHEM {- dot -} UCSD {- dot -} EDU
Date: 2008-03-03

Next message:
Subject: Re: Copper Staining
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-03-03