Re: [ccp4bb] Copper Staining

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Subject: Re: Copper Staining
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-03-03

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Subject: Re: Copper Staining
From: Dima Klenchin klenchin {- at -} FACSTAFF {- dot -} WISC {- dot -} EDU
Date: 2008-03-03

Subject: Re: Copper Staining
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-03-03

Touche'

Jacob

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-keller2@northwestern.edu
*******************************************

----- Original Message -----
From: "Artem Evdokimov"
To: "'Jacob Keller'" ;

Sent: Monday, March 03, 2008 6:31 PM
Subject: RE: [ccp4bb] Copper Staining

> There's an even faster reagent for staining - takes abut 1 minute overall
> and the results look just like dear old Coomassie. This reagent works
> wonderfully for us. Yes, the recipe is proprietary - but if you add up the
> cost of reagents for normal Coomassie and compare - this one isn't much
> more
> expensive.
>
> http://www.novexin.com/products/InstantBlue.html
>
> Artem
>
> P.S. I am not affiliated with Novexin in any way shape or form, but their
> gel stain does kick butt.
>
> -----Original Message-----
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Jacob
> Keller
> Sent: Monday, March 03, 2008 6:03 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] Copper Staining
>
> Dear Crystallographers:
>
> just to share something I found out recently, that is really helpful to
> know:
>
> 5 min Copper Stain protocol
>
> Normal SDS-PAGE gels can be stained in 5 min with 300mM CuCl2. Simply
> remove
>
> the gel, rinse ~10s in water, place 5 min in CuCl2, and the gel is
> negatively stained, with protein bands appearing clear, and background
> opaque white. To visualize, I put it on a flatbed scanner in a darkened
> room. It works in my hands every bit as well as coomassie (at least as
> sensitive, if not more), and if you want, you can stain afterwards in
> coomassie with the usual effect. To destain, place gel in EDTA. The
> original
>
> paper is Lee et al, Anal. Biochem. 1987. Instead of waiting hours, you can
> see the results in 5 min with copper! Also, there is no smell, and it can
> be
>
> re-used, to a point. Gels are stable in H2O for months, say Lee et al.
>
> Jacob
>
> p.s. I am not in copper comodities...
> p.p.s. if anybody knows of down-sides to this, please let me know.
>

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CCP4bb <-- 2008 <-- March 2008 <-- 03 March 2008

Previous message:
Subject: Re: Copper Staining
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-03-03

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Subject: Re: Copper Staining
From: Dima Klenchin klenchin {- at -} FACSTAFF {- dot -} WISC {- dot -} EDU
Date: 2008-03-03