Quick navigation:        Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |   
Protein structure
 

Re: [ccp4bb] Crosslinking reagents
 

Basic tutorials: 		 
 

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 04 March 2008
Previous message:
Subject: Re: phenix.refine and refmac
From: William Scott wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-03-04 		Next message:
Subject: Re: phenix.refine and refmac
From: yang li robertcatrukie {- at -} GMAIL {- dot -} COM
Date: 2008-03-04


Subject: Re: Crosslinking reagents
From: Nian Huang huangnian {- at -} GMAIL {- dot -} COM
Date: 2008-03-04
I will try that. But I don't think people can tell the final
concentration of glutaraldehyde in the drop by using its vapor. Maybe
using direct soaking is what I should do. By the way, should I quench
it by ammonium or not?

Nian Huang
Dept of Biochemistry
UT Southwestern Medical Center
Dallas, TX 75390


On Sun, Mar 2, 2008 at 12:28 AM, Nian Huang wrote:
> Dear all,
> I know most people use glutaraldehyde as the crosslinking reagent for
> their crystals. But there are a lot of other crosslinking chemicals
> available both in protein chemistry and histology, such as simple
> formaldehyde. Did anybody have experience with other chemicals and how
> did they compared with glutaradehyde? I have a crystal very sensitive
> to glutaradehyde but I really wants to try the crosslinking method.
> Thanks,
>
> Nian Huang
> Dept of Biochemistry
> UT Southwestern Medical Center
> Dallas, TX 75390
>
ProteinCrystallography.org: Copyright 2006-2007 by Quid United Ltd