Re: [ccp4bb] protein expression

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CCP4bb <-- 2008 <-- March 2008 <-- 04 March 2008

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From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2008-03-04

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Subject: Re: protein expression
From: wu donghui wdh0972 {- at -} GMAIL {- dot -} COM
Date: 2008-03-04

Hi Chen,

In this case, it seems that linker region is of great importance for the
proper folding of the two linked domains. I have not much experience in
linker region design, generally use (GS)5-10 times. However it depends on
individual case. Anyone who has successful experience in linker region
design might share your success with others. Thanks a lot.

Regards,

Donghui

On 3/5/08, Daniel Jin wrote:
>
> Hi,
>
> I have a protein with two independently folded domains. I can express
> either one in bacteria with pretty good expression yield. However, when I
> put them together with a linker, the expression drops significantly. I can
> barely see any soluble protein and most of it is now inclusion bodies. I
> used the same bacteria strain and expression/purification conditions. Is it
> normal? Any suggestion about how to improve? Many thanks.
>
> Best,
> Chen
>
>
>
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CCP4bb <-- 2008 <-- March 2008 <-- 04 March 2008

Previous message:
Subject: Re: phenix.refine and refmac
From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2008-03-04

Next message:
Subject: Problem with DM
From: yuhua yuhua {- dot -} lan {- at -} GMAIL {- dot -} COM
Date: 2008-03-04