[ccp4bb] (bigger) fragment identification of limited proteolysis w/ mass-spec

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Right Handedness of Density
From: Debanu Das ddas {- at -} LBL {- dot -} GOV
Date: 2007-03-30

Next message:
Subject: Re: (bigger) fragment identification of limited proteolysis w/ mass-spec
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-03-31

Subject: (bigger) fragment identification of limited proteolysis w/ mass-spec
From: Yong Tang liutang19 {- at -} GMAIL {- dot -} COM
Date: 2007-03-31

Dear all, just a super dummy question: I treated a protein with
trypsin, found the protein being degraded into two well-define
fragments, ran a sizing column to find them co-elute, sent the peak
fraction for mass-spec, got the two masses. Now here is the question -
is there any program readily available for me to roughly identify
these two (around 20K) fragments with the full-length sequence and
these two masses, and of cause, with the fact that I use trypsin to
cut it? I checked a lot of programs listed on Expasy to find them
mostly dealing with smaller peptide length. Any information would be
highly appreciated. Thanks and have a nice weekend, -yong

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Right Handedness of Density
From: Debanu Das ddas {- at -} LBL {- dot -} GOV
Date: 2007-03-30

Next message:
Subject: Re: (bigger) fragment identification of limited proteolysis w/ mass-spec
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-03-31