Re: [ccp4bb] Copper Staining

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Subject: Re: Copper Staining
From: Bjørn_Pañella_Pedersen bjopp {- at -} BIOXRAY {- dot -} AU {- dot -} DK
Date: 2008-03-05

One other staining method deserves to be mentioned with the others
already in this thread:
We use standard issue Coomassie Blue staining, but do the staining and
destaining with heated solutions (place the gel in a tray on a hotplate
set to ~90drg in a fumehood). We routinely have the result after 10-15
minutes (5 min stain, 5-10 min destain).

-bjørn
--
Bjørn Pañella Pedersen
Ph.D Student, MSc

Department of Molecular Biology Office: +45 89425021
University of Aarhus Lab: +45 89425010
Gustav Wieds Vej 10c Email: bpp@mb.au.dk
DK-8000 Aarhus C Lab WWW: http://www.bioxray.dk
Denmark

Jacob Keller wrote:
> Dear Crystallographers:
>
> just to share something I found out recently, that is really helpful to
> know:
>
> 5 min Copper Stain protocol
>
> Normal SDS-PAGE gels can be stained in 5 min with 300mM CuCl2. Simply
> remove the gel, rinse ~10s in water, place 5 min in CuCl2, and the gel
> is negatively stained, with protein bands appearing clear, and
> background opaque white. To visualize, I put it on a flatbed scanner in
> a darkened room. It works in my hands every bit as well as coomassie (at
> least as sensitive, if not more), and if you want, you can stain
> afterwards in coomassie with the usual effect. To destain, place gel in
> EDTA. The original paper is Lee et al, Anal. Biochem. 1987. Instead of
> waiting hours, you can see the results in 5 min with copper! Also, there
> is no smell, and it can be re-used, to a point. Gels are stable in H2O
> for months, say Lee et al.
>
> Jacob
>
> p.s. I am not in copper comodities...
> p.p.s. if anybody knows of down-sides to this, please let me know.

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Previous message:
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