Re: [ccp4bb] Copper Staining

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 05 March 2008

Previous message:
Subject: problem loading coot 0.4.1
From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-03-05

Next message:
Subject: Re: Removal of glycosylation sites in Picha expression construct
From: Stephen Weeks stephen {- dot -} weeks {- at -} VERIZON {- dot -} NET
Date: 2008-03-05

Subject: Re: Copper Staining
From: Mike Latchem mike {- dot -} latchem {- at -} DIAMOND {- dot -} AC {- dot -} UK
Date: 2008-03-05

You can also do this more quickly by microwaving your gels in
stain/destain solutions

just for the lazy or impatient

Regards

Mike

On Wed, 2008-03-05 at 12:09 +0100, Bjørn Pañella Pedersen wrote:
> One other staining method deserves to be mentioned with the others
> already in this thread:
> We use standard issue Coomassie Blue staining, but do the staining and
> destaining with heated solutions (place the gel in a tray on a hotplate
> set to ~90drg in a fumehood). We routinely have the result after 10-15
> minutes (5 min stain, 5-10 min destain).
>
> -bjørn

This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail.
Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd.
Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message.
Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 05 March 2008

Previous message:
Subject: problem loading coot 0.4.1
From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-03-05

Next message:
Subject: Re: Removal of glycosylation sites in Picha expression construct
From: Stephen Weeks stephen {- dot -} weeks {- at -} VERIZON {- dot -} NET
Date: 2008-03-05