[ccp4bb] Molecular replacement of a multidomain protein

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From: "Lunin, Vladimir" Vladimir_Lunin {- at -} NREL {- dot -} GOV
Date: 2008-03-06

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Date: 2008-03-07

Subject: Molecular replacement of a multidomain protein
From: Anjali Mehta anjali {- dot -} mht {- at -} GMAIL {- dot -} COM
Date: 2008-03-06

Dear All,
I am working with a Bifunctional protein of molecular weight ~60 kDa.
I have a 3.3 angstrom native dataset. The matthews number show there are 6
molecules in the asymmetric unit.
The structures of the individual domains are already known from prokaryotes.
The sequence identity with the known structures are about 30%.
I have tried molecular replacement using the two parts as models
respectively with CNS, MOLREP, PHASER etc. However I always get the solution
for one domain. I have also tried to fix that domain and find the other one.
But none of the programs can find a solution.
I am trying to model build the correct sequence of one domain using a
density modified (using CNS), NCS averaged (using RAVE) map but the map does
not look very good. The side chains are not clear. That might be due to the
fact that I am only having a partial model.
Any suggestion will be appreciated.
Thanks.
Ms. Anjali Mehta

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 06 March 2008

Previous message:
Subject: Postdoc position at NREL
From: "Lunin, Vladimir" Vladimir_Lunin {- at -} NREL {- dot -} GOV
Date: 2008-03-06

Next message:
Subject: 'Physicists and Biologists Workshop' at the CXS in Melbourne.
From: John R Helliwell jrhelliwell {- at -} GMAIL {- dot -} COM
Date: 2008-03-07