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Re: [ccp4bb] isopropanol as a precipitant
 

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CCP4bb <-- 2008 <-- March 2008 <-- 07 March 2008
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Subject: Re: Merging CCP4i projects from two computers
From: William Scott wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-03-07 		Next message:
Subject: MTZ cell troubles after sortmtz reindexing from P321 to C2
From: Pietro Roversi pietro {- dot -} roversi {- at -} PATH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-03-07


Subject: Re: isopropanol as a precipitant
From: Patrick Shaw Stewart Patrick {- at -} DOUGLAS {- dot -} CO {- dot -} UK
Date: 2008-03-07
Shivesh



It's often very difficult to harvest crystals from conditions that
contain isopropanol because the alcohol is volatile - especially with
50% or over!



A solution that has worked several times it so use the Vapor Batch
plates. http://www.douglas.co.uk/vb.htm



You can cover the drops with oil, and then put isopropanol solution into
the "moat" of the plate. The alcohol diffuses through the oil into the
drops, while the oil acts as a buffer, reducing loss of alcohol during
harvesting and freezing.



For a case study see http://www.douglas.co.uk/winner1.htm



Please let me know if anyone wants samples.



Patrick





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From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
shivesh kumar
Sent: 05 March 2008 07:23
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] isopropanol as a precipitant



Dear all
Sorry for non-CCP4 query.
I have crystallized a 7kDa protein in 50-60% of isopropanol,pH
4.0-4.6.The interesting thing is that the xtal appears within 5 hrs at
16 degree.
The protein conc is 5mg/ml and drop size is 3+1 with mother liquor 300
micro lt.Numerous xtals appears and but small is size.Do anyone can
share their experience with Isopropanol as a precipitant and to improve
the xtal quality with other precipitants.All suggestions are welcome.
Thanx in advance.
Shivesh


CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 07 March 2008
Previous message:
Subject: Re: Merging CCP4i projects from two computers
From: William Scott wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-03-07 		Next message:
Subject: MTZ cell troubles after sortmtz reindexing from P321 to C2
From: Pietro Roversi pietro {- dot -} roversi {- at -} PATH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-03-07


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