[ccp4bb] Nucleic acid refinement with Refmac at 3.6A

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CCP4bb <-- 2008 <-- March 2008 <-- 10 March 2008

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Subject: topology and parameter file
From: Surajit Banerjee sb_ju {- at -} YAHOO {- dot -} COM
Date: 2008-03-10

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Date: 2008-03-10

Subject: Nucleic acid refinement with Refmac at 3.6A
From: Alan Cheung cheung {- at -} LMB {- dot -} UNI-MUENCHEN {- dot -} DE
Date: 2008-03-10

Hi all - i'm trying to refine a protein-nucleic acid complex at 3.6A
resolution with refmac, with weight matrix 0.01. However, after
restrained refinement, many of the base pairs are no longer co-planar.
Are there watson-crick restraints in refmac, or some other restraint
keyword that might help? Or should i be using CNS?

Alan

--
Alan Cheung
Gene Center
Ludwig-Maximilians-University
Feodor-Lynen-Str. 25
81377 Munich
Germany
Phone: +49-89-2180-76845
Fax: +49-89-2180-76999
E-mail: cheung@lmb.uni-muenchen.de

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 10 March 2008

Previous message:
Subject: topology and parameter file
From: Surajit Banerjee sb_ju {- at -} YAHOO {- dot -} COM
Date: 2008-03-10

Next message:
Subject: Postdoctoral Position in Structural Biology of the Cell Surface Receptors
From: ±è °æ±Ô kkim {- at -} MED {- dot -} SKKU {- dot -} AC {- dot -} KR
Date: 2008-03-10