Re: [ccp4bb] Color of heme containing Xtals

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Subject: Color of heme containing Xtals
From: Jan Schoepe j {- dot -} schoepe {- at -} YAHOO {- dot -} DE
Date: 2008-03-12

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Subject: Re: Color of heme containing Xtals
From: "Sanishvili, Ruslan" rsanishvili {- at -} ANL {- dot -} GOV
Date: 2008-03-12

Subject: Re: Color of heme containing Xtals
From: Ian Ollmann iano {- at -} APPLE {- dot -} COM
Date: 2008-03-12

On Mar 12, 2008, at 12:48 PM, Jan Schoepe wrote:

> Hello everybody,
>
> I wonder if anybody has experience with heme (or to be more precise:
> heme b) containing proteins which Xtals do not look red under the
> microscope. How might the technique for crystallization (e.g.
> sitting drop, hanging drop) influence the intensity of the color?
> Many thanks!

If there is no metal in the heme, its color will be very different.
If your crystallization buffer contains EDTA or other strong chelating
agents, I imagine this might happen, though protein crystallization is
not my area.

Ian

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CCP4bb <-- 2008 <-- March 2008 <-- 12 March 2008

Previous message:
Subject: Color of heme containing Xtals
From: Jan Schoepe j {- dot -} schoepe {- at -} YAHOO {- dot -} DE
Date: 2008-03-12

Next message:
Subject: Re: Color of heme containing Xtals
From: "Sanishvili, Ruslan" rsanishvili {- at -} ANL {- dot -} GOV
Date: 2008-03-12