[ccp4bb] 2-D crystallization of membrane proteins

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From: Peter Zwart PHZwart {- at -} LBL {- dot -} GOV
Date: 2008-03-13

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From: byron delabarre byron {- dot -} ccp4 {- at -} GMAIL {- dot -} COM
Date: 2008-03-13

Subject: 2-D crystallization of membrane proteins
From: gxzhao gxzhao {- at -} FJIRSM {- dot -} AC {- dot -} CN
Date: 2008-03-13

Hi,
everyone, who has much more experience about the 2-D crystallization of membrane proteins using the dialysis method.

1) how to avoid the change of whole sample's volume after dialysis?
2) can you tell me some tricks during making grids, that is, when we put dialysis sample on grids, your experiences?

Thanks in advance, Thanks your kindness;

best regards,

gxzhao

lab of electron cryo-microscopy
school of biology
Georgia institute of technology
Atlanta, GA 30318

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 13 March 2008

Previous message:
Subject: Beam Time at BCSB beamlines in Berkeley
From: Peter Zwart PHZwart {- at -} LBL {- dot -} GOV
Date: 2008-03-13

Next message:
Subject: coot extensions for residue mutation to Cbeta
From: byron delabarre byron {- dot -} ccp4 {- at -} GMAIL {- dot -} COM
Date: 2008-03-13